Add to favorites Favorited View favorites

Job Description Summary

A cancer research laboratory in the Hollings Cancer Center and Darby Children’s Research Institute has a job opening for a Graduate Research Assistant. The lab uses in vitro, ex vivo and in vivo models to elucidate the intracellular drivers of pediatric brain tumor growth. The research will particularly focus on using genetic mouse models and patient derived orthotopic xenografts to dissect the drivers of medulloblastoma tumor growth and relapse. Studies will use a range of complementary methods including cellular and molecular biology, IHC and IF imaging, flow cytometry methods, single-cell RNA seq, and functional cell-based assays.

Entity

Medical University of South Carolina (MUSC - Univ)

Worker Type

Employee

Worker Sub-Type​

Temporary

Cost Center

CC001013 COM PEDS Children's Research Institute CC

Pay Rate Type

Hourly, Salary

Pay Grade

University-00

Pay Range

0.00 - 0.00 - 0.000

Scheduled Weekly Hours

40

Work Shift

Job Description

A cancer research laboratory in the Hollings Cancer Center and Darby Children’s Research Institute has a job opening for a Graduate Research Assistant. The lab uses in vitro, ex vivo and in vivo models to elucidate the intracellular drivers of pediatric brain tumor growth. The research will particularly focus on using genetic mouse models and patient derived orthotopic xenografts to dissect the drivers of medulloblastoma tumor growth and relapse. Studies will use a range of complementary methods including cellular and molecular biology, IHC and IF imaging, flow cytometry methods, single-cell RNA seq, and functional cell-based assays.

Job Duties:

35% - Perform a number of cell and molecular biology procedures. Preparing cells from the mouse models, cell culture, molecular (cell viability, IF, FACs analyses, immunoblotting, PCR, qPCR, reporter assays, IHC, ChIP…), and functional genomic evaluation of gene expression and transcription factor binding to target genes, including single cell RNAseq, standard RNAseq and ChIPseq. Bacterial recombinant DNA work and lentiviral production for in vitro transfection and transduction experiments. - (Essential)

35% - Manage multiple projects with minimal guidance from PI. Responsibilities include designing, setting-up and running experiments in consultation with the Principal Investigator and effectively recording, analyzing, acquiring and analysis. The PI provides an overview of the hypothesis and overall plans. - (Essential)

20% - Complete xenograft and allograft mouse tumor models, including implantation of tumor cells in the brain, compound dosing, and in vivo tumor growth assessment. Tissue and cell isolation from mice, followed by immunohistochemistry, microscopy and a variety of cellular and molecular biology procedures. - (Essential)

10% - Participate in laboratory and scientific meetings, and manuscript and grant preparation. - (Essential)

Additional Job Description

Physical Requirements: (Note: The following descriptions are applicable to this section: Continuous - 6-8 hours per shift; Frequent - 2-6 hours per shift; Infrequent - 0-2 hours per shift) Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32° F to 100° F) when required due to assignments in cold rooms and/or foreign countries. (Infrequent) Ability to work in confined/cramped spaces. (Continuous) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Frequent) Ability to fully use both legs. (Frequent) Ability to reach in all directions, stretch, bend, while moving items/materials from both sitting and standing positions. (Frequent) Ability to maintain good olfactory sensory function/distinction. (Continuous) Ability to lift materials up to 20 lbs., unassisted. (Infrequent) Ability to lift and carry 4 liter waste material bottles a distance of 30 feet, unassisted. (Frequent) Ability to lift and carry 25-30 lbs. jugs of liquid a distance of 100 feet, unassisted. (Infrequent) Ability to lift file boxes, from floor level to counter tops, unassisted. (Frequent) Ability to push/ Ability to lift 40 lbs. centrifuge rotors from floor level to height of 40 inches, then down 20 inches into centrifuge drum, unassisted. (Infrequent) Ability to lift 25 lbs. boxes, from floor level to maximum height of 72 inches, unassisted. (Infrequent) Ability to push/pull file boxes from one location to another at floor level. (Frequent) Ability to maneuver heavy equipment on bench tops, 50 lbs. or greater, unassisted. (Frequent) Ability to perform gross motor functions with frequent fine motor movements. (Continuous) Ability to maintain 20/40 vision, corrected, in one eye or with both eyes. (Continuous) Ability to match or discriminate between colors, normally. (Continuous) Ability to hear and recognize various alarms emitting from work area systems. (Continuous) Ability to hear and/or understand conversations. (Continuous) Ability to work in a darkroom for processing film, etc. (Continuous) Ability to be qualified physically for respirator use, initially and annually. (Continuous) Additional New Requirements: Ability to work rotating shifts as required. Ability to work overtime as required. Ability to learn and use new processes, tools and equipment as required.

If you like working with energetic enthusiastic individuals, you will enjoy your career with us!

The Medical University of South Carolina is an Equal Opportunity Employer. MUSC does not discriminate on the basis of race, color, religion or belief, age, sex, national origin, gender identity, sexual orientation, disability, protected veteran status, family or parental status, or any other status protected by state laws and/or federal regulations. All qualified applicants are encouraged to apply and will receive consideration for employment based upon applicable qualifications, merit and business need.

Medical University of South Carolina participates in the federal E-Verify program to confirm the identity and employment authorization of all newly hired employees. For further information about the E-Verify program, please click here: http://www.uscis.gov/e-verify/employees